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11.
摘要:目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。  相似文献   
12.
Based on statistical data and population flow data for 2016,and using entropy weight TOPSIS and the obstacle degree model,the centrality of cities in the Yangtze River Economic Belt(YREB)together with the factors influencing centrality were measured.In addition,data for the population flow were used to analyze the relationships between cities and to verify centrality.The results showed that:(1)The pattern of centrality conforms closely to the pole-axis theory and the central geography theory.Two axes,corresponding to the Yangtze River and the Shanghai-Kunming railway line,interconnect cities of different classes.On the whole,the downstream cities have higher centrality,well-defined gradients and better development of city infrastructure compared with cities in the middle and upper reaches.(2)The economic scale and size of the population play a fundamental role in the centrality of cities,and other factors reflect differences due to different city classes.For most of the coastal cities or the capital cities in the central and western regions,factors that require long-term development such as industrial facilities,consumption,research and education provide the main competitive advantages.For cities that are lagging behind in development,transportation facilities,construction of infrastructure and fixed asset investment have become the main methods to achieve development and enhance competitiveness.(3)The mobility of city populations has a significant correlation with the centrality score,the correlation coefficients for the relationships between population mobility and centrality are all greater than 0.86(P<0.01).The population flow is mainly between high-class cities,or high-class and low-class cities,reflecting the high centrality and huge radiating effects of high-class cities.Furthermore,the cities in the YREB are closely linked to Guangdong and Beijing,reflecting the dominant economic status of Guangdong with its geographical proximity to the YREB and Beijing's enormous influence as the national political and cultural center,respectively.  相似文献   
13.
ABSTRACT

Pedestrian networks play an important role in various applications, such as pedestrian navigation services and mobility modeling. This paper presents a novel method to extract pedestrian networks from crowdsourced tracking data based on a two-layer framework. This framework includes a walking pattern classification layer and a pedestrian network generation layer. In the first layer, we propose a multi-scale fractal dimension (MFD) algorithm in order to recognize the two different types of walking patterns: walking with a clear destination (WCD) or walking without a clear destination (WOCD). In the second layer, we generate the pedestrian network by combining the pedestrian regions and pedestrian paths. The pedestrian regions are extracted based on a modified connected component analysis (CCA) algorithm from the WOCD traces. We generate the pedestrian paths using a kernel density estimation (KDE)-based point clustering algorithm from the WCD traces. The pedestrian network generation results using two actual crowdsourced datasets show that the proposed method has good performance in both geometrical correctness and topological correctness.  相似文献   
14.

The use of spontaneous potential (SP) anomalies is well known in the geophysical literatures because of its effectiveness and significance in solving many complex problems in mineral exploration. The inverse problem of self-potential data interpretation is generally ill-posed and nonlinear. Methods based on derivative analysis usually fail to reach the optimal solution (global minimum) and trapped in a local minimum. A new simple heuristic solution to SP anomalies due to 2D inclined sheet of infinite horizontal length is investigated in this study to solve these problems. This method is based on utilizing whale optimization algorithm (WOA) as an effective heuristic solution to the inverse problem of self-potential field due to a 2D inclined sheet. In this context, the WOA was applied first to synthetic example, where the effect of the random noise was examined and the method revealed good results using proper MATLAB code. The technique was then applied on several real field profiles from different localities aiming to determine the parameters of mineralized zones or the associated shear zones. The inversion parameters revealed that WOA detected accurately the unknown parameters and showed a good validation when compared with the published inversion methods.

  相似文献   
15.
Huang  Mengqian  Zhang  Lei  Zhang  Cun  Chen  Shuai 《Natural Resources Research》2020,29(3):1687-1704

The factors affecting permeability change under repeated mining of coal seams are important study aspects that need to be explored. This study combined various stress variation characteristics of protective seam mining and simplified the stress path of repeated mining in protective seam mines. Based on the results from the bespoke gas flow and displacement testing apparatus, seepage tests for simulated repetitive mining were carried out. The results simulated the actual behavior very well. With any drastic increase in the mining influence, the axial deviation stress in the stress path increased, and the greater the difference in coal permeability during the unloading and stress recovery stage, the more substantial the increase in permeability. The change in coal permeability was significantly influenced by the severity of simulated repeated mining cycles. When the mining stress exceeded a critical value, the permeability of the coal sample increased with the increase in the number of loading and unloading cycles, but the reverse was true when the mining stress was lower than the critical value. The effective sensitivity of seepage to the applied stress decreased with an increase in the number of stress cycles. With a decrease in the deviation stress, that is, with lower severity of mining influence, the effective sensitivity of coal seepage to stress gradually decreased.

  相似文献   
16.
Natural Resources Research - In the past few decades, a variety of data-driven predictive modeling techniques has led to a dramatic advancement in mineral prospectivity mapping (MPM). The random...  相似文献   
17.
Yu  Zhi  Shi  Xiuzhi  Zhou  Jian  Chen  Xin  Miao  Xiaohu  Teng  Bing  Ipangelwa  Timo 《Natural Resources Research》2020,29(2):843-865
Natural Resources Research - A large ore loss and dilution can be expected when using a pre-blast ore boundary for shovel guidance because of the movement and re-distribution of ore in the muck...  相似文献   
18.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   
19.
A decision support process is presented to accommodate selecting and scaling of earthquake motions as required for the time domain analysis of structures. Code-compatible suites of seismic motions are provided being, at the same time, prequalified through a multi-criterion approach to induce response parameters with reduced variability. The latter is imperative to increase the reliability of the average response values, normally required for the code-prescribed design verification of structures. Structural attributes like the dynamic characteristics as well as criteria related to variability of seismic motions and their compliance with a target spectrum are quantified through a newly introduced index, δ svsc , which aims to prioritize motions suites for response history analysis. To demonstrate the applicability of the procedure presented, the structural model of a multi-story building was subjected to numerous suites of motions that were highly ranked according to both the proposed approach (δ svsc ) and the conventional one (δ conv ), that is commonly used for earthquake records selection and scaling. The findings from numerous linear response history analyses reveal the superiority of the proposed multi-criterion approach, as it extensively reduces the intra-suite structural response variability and consequently, increases the reliability of the design values. The relation between the target reliability in assessing structural response and the size of the suite of motions selected was also investigated, further demonstrating the efficiency of the proposed selection procedure to achieve higher response reliability levels with smaller samples of ground motion.  相似文献   
20.
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